CD47 Recombinant Protein

ProSci
Product Code: PSI-96-849
Product Group: Recombinant Proteins
Supplier: ProSci

CodeSizePrice
PSI-96-849-0.1mg0.1mg£657.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.

Documents

Further Information

Additional Names:
CD47,MER6,IAP,OA3
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Background:
Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
Background References:
  • Lindberg F.P., et al., 1993, J. Cell Biol. 123:485-496.
  • Latour S., et al., 2001, J. Immunol. 167:2547-2554.
  • Piccio L., et al., 2005, Blood 105:2421-2427.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human SIRP alpha (HPLC-verified), His Tag at 2 ug/mL (100 uL/well) can bind Human CD47, mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.5-8 ng/mL.
Buffer:
50 mM Tris, 100 mM Glycine, pH7.5
Fusion Tag:
mouse IgG2a Fc tag
NCBI Gene ID #:
961
NCBI Official Name:
leukocyte surface antigen CD47 isoform 2 precursor
NCBI Official Symbol:
CD47
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
40.8 kDa
Protein Accession #:
NP_942088
Purity:
>95% as determined by SDS-PAGE.

Endotoxin level is less than 0.1 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
Q08722