B7-1 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-970-0.1mg | 0.1mg | £560.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Background:
B7-1 and B7-2, together with their receptors CD28 and CTLA?4, constitute one of the dominant co-stimulatory pathways that regulate T? and B?cell responses. Although both CTLA?4 and CD28 can bind to the same ligands, CTLA?4 binds to B7?1 and B7?2 with a 20 ? 100 fold higher affinity than CD28 and is involved in the down?regulation of the immune response.
B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-kappaB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
Background References:
- Peach RJ., et al., 1995, J. Biol. Chem. (UNITED STATES) 270 (36): 21181?7.
- Stamper CC., et al., 2001, Nature (England) 410 (6828): 608?11.
- Bhatia S., et al., 2005, Proc. Natl. Acad. Sci. 102: 15569-15574.
- Khan N., et al., 2007, Int. Immunol. 19:477-486.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Mouse B7-1, Fc Tag at 2 ug/mL (100 ul/well),can bind Mouse CTLA-4, His Tag with a linear range of 0.02-0.8 ng/mL.
Buffer:
Tris?with?Glycine,?Arginine?and?NaCl,?pH7.5
Fusion Tag:
Fc Tag
NCBI Gene ID #:
12519
NCBI Official Name:
T-lymphocyte activation antigen CD80
NCBI Official Symbol:
B7-1
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
50.3 kDa
Protein Accession #:
Q00609-1
Purity:
>95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
Q00609-1