Dkk-1 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-990-0.05mg | 0.05mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
DKK1,SK
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK1 takes part in embryonic development through its inhibition of the WNT signaling pathway, binds to LRP6 with high affinity and prevents the Frizzled-Wnt-LRP6 complex formation in response to Wnts. DKK1 promotes LRP6 internalization and degradation when it forms a ternary complex with the cell surface receptor Kremen.DKK1 not olny functions as a head inducer during development, but also regulates joint remodeling and bone formation, which suggests roles for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma. More recently research reported, DKK1 impacts eye development from a defined developmental time point on, and is critical for lens separation from the surface ectoderm via beta-catenin mediated Pdgfralpha and E-cadherin expression.
Background References:
- Li Y, Lu W, et al., 2010, PLoS One.Jun 8;5 (6) :e11014.
- Yamaguchi Y, Morita A, et al., 2009, J Investig Dermatol Symp Proc. Aug;14 (1) :73-5.
- Lieven O, R?ther U.et al., 2011, Dev Biol. Apr 22.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
13380
NCBI Official Name:
Dickkopf-related protein 1
NCBI Official Symbol:
Dkk-1
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
28 kDa
Protein Accession #:
O54908
Purity:
>95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
O54908