14-3-3 sigma Antibody

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NSJ Bioreagents
Product Code: NSJ-R30895
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents

CodeSizePrice
NSJ-R30895-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the 14-3-3 sigma antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human esophagus squama cancer with 14-3-3 sigma antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC-P: 14-3-3 sigma antibody testing of human tonsil tissue
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IHC-P: 14-3-3 sigma antibody testing of human tonsil tissue
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IHC-P: 14-3-3 sigma antibody testing of human oesophagus squama cancer tissue
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IHC staining of FFPE human intestinal cancer with 14-3-3 sigma antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human breast cancer with 14-3-3 sigma antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human U-2 OS cells with 14-3-3 sigma antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with 14-3-3 sigma antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
9 / 10
Western blot testing of 1) human HeLa, 2) human PC-3, 3) rat brain and 4) mouse brain lysate with 14-3-3 sigma antibody. Predicted molecular weight: ~28 kDa.
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Flow cytometry testing of human U-2 OS cells with 14-3-3 sigma antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 14-3-3 sigma antibody.
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IHC staining of FFPE human esophagus squama cancer with 14-3-3 sigma antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC-P: 14-3-3 sigma antibody testing of human tonsil tissue
IHC-P: 14-3-3 sigma antibody testing of human tonsil tissue
IHC-P: 14-3-3 sigma antibody testing of human oesophagus squama cancer tissue
IHC staining of FFPE human intestinal cancer with 14-3-3 sigma antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with 14-3-3 sigma antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with 14-3-3 sigma antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with 14-3-3 sigma antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human PC-3, 3) rat brain and 4) mouse brain lysate with 14-3-3 sigma antibody. Predicted molecular weight: ~28 kDa.
Flow cytometry testing of human U-2 OS cells with 14-3-3 sigma antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 14-3-3 sigma antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the 14-3-3 sigma antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Stratifin (SFN), also known as 14-3-3 sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al.(1993) obtained a peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein called stratifin was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 140-156 (KKRIIDSARSAYQEAMD) were used as the immunogen for this 14-3-3 sigma antibody (100% homologous in human, mouse and rat).
Limitation:
This 14-3-3 sigma antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P31947