14-3-3 sigma Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4072
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4072-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the 14-3-3 sigma antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) 22RV1, 5) rat lung, 6) rat PC-12, 7) mouse lung, 8) mouse HEPA1-6 and 9) mouse NIH3T3 lysate with 14-3-3 sigma antibody at 0.5ug/ml.
2 / 5
IF/ICC staining of FFPE human A431 cells with 14-3-3 sigma antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 5
IF/ICC staining of FFPE human A549 cells with 14-3-3 sigma antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE human rectal cancer with 14-3-3 sigma antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 5
Flow cytometry testing of human U-2 OS cells with 14-3-3 sigma antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 14-3-3 sigma antibody.
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Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) 22RV1, 5) rat lung, 6) rat PC-12, 7) mouse lung, 8) mouse HEPA1-6 and 9) mouse NIH3T3 lysate with 14-3-3 sigma antibody at 0.5ug/ml.
IF/ICC staining of FFPE human A431 cells with 14-3-3 sigma antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IF/ICC staining of FFPE human A549 cells with 14-3-3 sigma antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with 14-3-3 sigma antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of human U-2 OS cells with 14-3-3 sigma antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 14-3-3 sigma antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunofluorescence/Immunocytochemistry: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the 14-3-3 sigma antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Stratifin (SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human partial protein corresponding to amino acids M1-S248 was used as the immunogen for the 14-3-3 sigma antibody.
Limitation:
This 14-3-3 sigma antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P31947