17-beta-Hydroxysteroid dehydrogenase 4 Antibody (HSD17B4)

NSJ Bioreagents
Product Code: NSJ-R30817
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents

CodeSizePrice
NSJ-R30817-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the 17-beta-Hydroxysteroid dehydrogenase 4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 17-beta-Hydroxysteroid dehydrogenase 4 antibody and Lane 1: mouse heart; 2: rat heart; 3: human placenta 4: human MCF7 lysate. Predicted molecular weight ~80 kDa.
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IHC-P: 17-beta-Hydroxysteroid dehydrogenase 4 antibody testing of human lung cancer tissue
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IHC-P: 17-beta-Hydroxysteroid dehydrogenase 4 antibody testing of human intestinal cancer tissue

Western blot testing of 17-beta-Hydroxysteroid dehydrogenase 4 antibody and Lane 1: mouse heart; 2: rat heart; 3: human placenta 4: human MCF7 lysate. Predicted molecular weight ~80 kDa.
IHC-P: 17-beta-Hydroxysteroid dehydrogenase 4 antibody testing of human lung cancer tissue
IHC-P: 17-beta-Hydroxysteroid dehydrogenase 4 antibody testing of human intestinal cancer tissue

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the 17-beta-Hydroxysteroid dehydrogenase 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The HSD17B4 gene encodes an enzyme involved in peroxisomal fatty acid beta-oxidation. It was first identified as a 17-beta-estradiol dehydrogenase. Peroxisomal beta-oxidation of fatty acids is catalyzed by 3 enzymes: acyl-CoA oxidase; the D-bifunctional enzyme with enoyl-CoA-hydratase and D-3-hydroxyacyl-CoA dehydrogenase activity, and 3-ketoacyl-CoA thiolase. The D- and L-bifunctional proteins have different substrate specificities. The D-bifunctional protein catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids and also acts in shortening cholesterol for bile acid formation. In contrast, the L-specific bifunctional protein does not have the latter 2 activities.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human HSD17B4 (NIMLSQKLQMILKDYAKL) was used as the immunogen for this 17-beta-Hydroxysteroid dehydrogenase 4 antibody (100% homologous in human, mouse and rat).
Limitation:
This 17-beta-Hydroxysteroid dehydrogenase 4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
F5HE57