5HT1A Receptor Antibody

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NSJ Bioreagents
Product Code: NSJ-R30731
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30731-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the 5HT1A Receptor antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human lung cancer tissue with 5HT1A Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human endometrial adenocarcinoma tissue with 5HT1A Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE rat lymph tissue with 5HT1A Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 4
Western blot testing of 1) human HCCT and 2) rat liver tissue lysate with 5HT1A Receptor antibody. Predicted molecular weight ~46 kDa.
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IHC staining of FFPE human lung cancer tissue with 5HT1A Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrial adenocarcinoma tissue with 5HT1A Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat lymph tissue with 5HT1A Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HCCT and 2) rat liver tissue lysate with 5HT1A Receptor antibody. Predicted molecular weight ~46 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the 5HT1A Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
5HT1AR is a subtype of 5HTR that binds the endogenous neurotransmitter serotonin. It is a G protein-coupled receptor (GPCR) that is coupled to Gi/Go and mediates inhibitory neurotransmission. The HTR1A gene is located at 5q12.3. The decreases in 5HT1AR densities correlated with decreased glucose utilization as measured by PET scan. Activation of 5HT1ARs has been demonstrated to impair cognition, learning, and memory by inhibiting the release of glutamate and acetylcholine in various areas of the brain. In the dorsal raphe nucleus they are co-localized with neurokinin 1(NK1) receptors and have been shown to inhibit the release of substance P, their endogenous ligand.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human 5HT1A Receptor(NKDFQNAFKKIIKCKFCRQ) was used as the immunogen for this 5HT1A Receptor antibody.
Limitation:
This 5HT1A Receptor antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P08908