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Methylation

DNA methylation is a major epigenetic modification involving the addition of a methyl group to the 5 position of cytosine by DNA methyltransferase to form 5-methylcytosine (5-mC). This epigenetic mark has the power to turn genes “on” or “off” and it plays an important role in normal human development, aging, tumorigenesis, and other genetic and epigenetic diseases. Similarly, DNA hydroxymethylation, caused by oxidation of 5-mC through the TET family of enzymes, was further discovered to be involved in controlling gene expression and is a key DNA demethylation process as part of the DNA methylation cycle.

Histone methylation causes transcription repression or activation, depending on the target sites. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcription repression or activation. Measurement of histone methyltransferase activity and quantification of histone methylation patterns have become pivotal in studying epigenetic regulation of genes, as well as inhibitor discovery.

Caltag Medsystems provides a wide range of quantification assays, inhibitor screening kits, isolation kits, highly validated antibodies, recombinant proteins, inhibitors and more for the study of DNA methylation, RNA methylation and histone methylation & demethylation.