Alkaline Phosphatase (ALP) Activity Assay Kit Video
This video demonstrates how to perform a colorimetric Alkaline Phosphatase activity assay in biological samples using a simple, highly sensitive, add-incubate-read protocol. The results are quantitative and reproducible.
Glucose Assay Kit Protocol (Colorimetric) Video
This BioVision Video features our Glucose Assay Kit (K606-100) : High-Throughput Suitable Assay to Measure Glucose in a variety of biological samples such as serum, plasma & other body fluids, food, growth media, fermentation, glucose feeding in protein expression processes etc. Rapid, Simple & Sensitive. Detection Range: 1-10000 μM. 100 Assays.
Glucose Assay Kit Protocol (Fluorometric) Video
This BioVision Video features our Glucose Assay Kit (K606-100): High-Throughput Suitable Assay to Measure Glucose in a variety of biological samples such as serum, plasma & other body fluids, food, growth media, fermentation, glucose feeding in protein expression processes etc. Rapid, Simple & Sensitive. Detection Range: 1-10000 μM. 100 Assays.
PicoProbe™ Free Glycerol Fluorometric Assay Kit Video
This BioVision Video features our PicoProbe™ Free Glycerol Fluorometric Assay Kit (K643-100): The most sensitive kit on the market to measure free glycerol. Rapidly measures free glycerol in tissues, adherent & suspension cells as well as in biological fluids such as serum, plasma etc. Detection limit: less than 40 pmol. 100 Assays.
Urea Colorimetric Assay Kit Video
This BioVision Video features our newest Urea Colorimetric Assay Kit (K376-100) being used to measure Urea in human urine sample. This kit is fast, sensitive & easy to use.
NCI - Moving Forward: The Critical Importance of High-Quality Biospecimens in Cancer Research
Hear what important cancer community stakeholders from the patient, oncology, research and drug development communities have to say about the critical importance of high-quality biospecimens and biorepositories for cancer research and patient care.
Thawing and Plating Cryopreserved Cells
A tutorial on how to properly thaw and plate cryopreserved cells from ZenBio
Cell Proliferation Assay Kit Video
This video demonstrates the step-wise process of measuring cell proliferation in response to growth factors/cytokines/mitogens or nutrients. BioVision's assay is faster, more stable, and more sensitive than MTT, XTT, MTS based assays.
How Flow Cytometry Can Help Advance Your Research
This webinar provides an introduction to flow cytometry, a technique that uses fluorescently conjugated antibodies and dyes to identify characteristics in heterogeneous populations of cells. In addition we will present case studies on how flow cytometry can be used in drug discovery.
HIT Alert Kit
A complete kit for the Reliable Diagnosis of Heparin-Induced Thrombocytopenia
MBL has incorporated the patented mutation of the a3 domain in their HLA class I Tetramers and is the only manufacturer of MHC multimers with this technology to enhance specificity for the antigen-specific T cell receptor.
Smart-IP: Tag antibodies conjugated to magnetic beads for immunoprecipitation
MBL International Corporation offers a line of highly specific antibodies conjugated to magnetic beads. This method is an advantage over conventional immunoprecipitation of tagged proteins because it eliminates time consuming centrifugation steps. The magnetic beads are covalently bound to a Tag antibody and the beads are easily isolated using a magnetic rack.
Antibody Purification Video
This video shows you the steps for purifying a specific antibody from serum using BioVision’s high binding Protein A/Protein G-Sepharose beads. The purified antibody can then be used for Western Blotting, Immunoprecipitation and many other applications.
Cell Cryopreservation Video
This BioVision Video demonstrates how to Trypsinize, count and freeze adherent mammalian cells for future use. These cryopreserved cells can be maintained in Liquid Nitrogen and thawed as needed.
Cell Splitting Video
This short video will take you through the steps (Trypsinization, counting and calculations) involved in passaging confluent adherent mammalian cells for future experiments or for maintenance of cell lines using Good Lab Practices and sterile technique.
Watch this video demonstration showing you how to remove small molecules (salts, surfactants, solvents, detergents) from any sample by Dialysis using BioVision’s DiaEasy™ Dialyzer line of products.
Protein Quantitation Video
Assays to determine protein concentration in a sample are many and the choices can become confusing. This video includes a guide to help you choose the right assay based on sample characteristics and a step-wise protocol for a colorimetric (OD 562 nm) detergent-compatible BCA assay with sample dilution guidelines.
Tissue Homogenization Video
This video demonstrates how to prepare a homogenate from animal tissue using a Dounce homogenizer. The tissue homogenate can then be used for many different downstream applications like western blotting, enzyme assays etc. Similar technique may also be applied to cells for preparation of cell lysates.
Cell Culture (Attached Cell)
Cell culture is the process by which cells are grown under controlled conditions. This video show you how to culture attached cells from cell thrawing, maintain, to cell freezing.
In competitive ELISA, unlabeled antibody is incubated in the presence of its antigen. Then these bound antibody/antigen complexes are then added to an antigen coated well. After washing, unbound antibodies are removed. The more analytes in the sample, the less antibodies will be able to bind to antigens in the well. The signal is then detected using labeled secondary antibodies and the decrease in signal is compared to a control. The major advantage of a competitive ELISA is the ability to use crude or impure samples and still selectively bind any antigen that may be present.
Immunofluorescence is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope.
Immunohistochemistry is a method of detecting the presence of specific proteins in cells or tissues. It is widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.
The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample. In the assay, the antigen of interested is immobilized by direct adsorption to the assay plate. Detection of the antigen can then be performed by using a matched set of primary antibody and conjugated secondary antibodies.
Polymerase Chain Reaction
Polymerase Chain Reaction (PCR) is a technique to amplify few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. There are three major steps (Denaturation, Annealing and Extension) in a PCR and repeat for 30 or 40 cycles
Real-time PCR, also called quantitative real time PCR (Q-PCR/qPCR), is used to amplify and simultaneously quantify a targeted DNA molecule. It enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample.
Reverse Transcription PCR
Reverse transcription PCR includes two steps. The first step is reverse transcription, in which RNA is reverse transcribed to its DNA complement (complementary DNA, or cDNA) using reverse transcriptase and primers. The second step is amplification using traditional or real-time PCR.
Western Blotting is an analytical technique used to detect specific proteins in a given sample. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane where they are detected using antibodies specific to the target protein.
H&E stain is a popular staining method in histology. It's a combination of two dyes: the basic dye (hematoxylin) and the alcohol-based dye (eosin). In an H&E stain you'll usually see both eosinophilia and basophilia: the nuclei of cells basophilic (blue), while eosinophilia is typical of cytoplasmic constituents (pink).
Educational video for the RIP-Chip assay technology for microRNA. Identify miRNAs bound to their mRNA targets.
3Helix - Assessing Mechanical Damage of Collagen with CHPs
Collagen is the major building block in all load bearing tissues including tendon, ligament, bone, and cartilage. Mechanical injury to these tissues causes changes in collagen structure which can initiate pathological processes without detectable morphological changes at the macroscale. Using CHPs you can now visualize the mechanical damage to the triple helix.
3Helix - Fibrotic Tissue Staining with CHP
Collagen hybridizing peptides allow for visualization of fibrotic tissue or any tissues with an increase of collagen turnover.
3Helix - How Collagen Hybridizing Peptides Work
Collagen has a unique triple helical structure that is unfolded in tissues during diseases, development, mechanical injury, and decellularization. 3Helix commercializes labeled collagen hybridizing peptide (CHP) that specifically binds to such unfolded collagen chains.
3Helix - Quantifying Collagen Damage After Tissue Decellularization
Every decellularization method may alter the composition and the micro-architecture of ECM, causing variations of the biological activity and mechanical integrity of the decellularized material. CHP specifically detects denatured collagen in the decellularized tissue/organ, enabling assessment of the effect of decellularization upon ECM at the molecular level.
3Helix - Skeletal Imaging with Collagen Hybridizing Peptides
The skeletal system has a large quantity of collagen, making visualization of skeletal development, disease and turnover with CHPs a perfect use case. Find out more at 3helix.com
Chicken Myocyte Primary Culture
Primary culture of chick embryo myocyte culture can be used for the detection and assessment of drug responses on cardiomyocyte.
Product How To...
Operating Steps for Cloud-Clone ELISA Kits
Information on the storage and use of ELISA Kits, provided by Cloud-Clone Corp. - distributed in the UK by Caltag Medsystems.