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Cell Culture

Best Practices for MNC Isolation: Techniques by Tissue Type and When to Use Them

In this webinar we will discuss best practices for MNC isolation for several types of tissue based on researcher needs. We will outline techniques as they vary by cost, timing, ease, viability and availability. The webinar will conclude with examples of case studies on customized isolation projects provided by AllCells. Topics to be covered in the webinar: Intro Whole Blood Bone Marrow/Cord Blood Leuko Paks (Mobilized/Non-mobilized) Custom Isolation Case Studies Q&A

Case study: In vitro human primary cell based screening assays for vaccine adjuvant development

Once matured, Dendritic cells (DC) are capable of initiating and propagating an antigen-specific adaptive immune response. DC based immunotherapy is a promising treatment alternative for various solid tumor and hematological malignancies, an adaptive DC based response is also a goal for effective vaccines. Identifying novel, immunostimulatory drug candidates that can stimulate DC maturation is essential for development of such immunotherapies. In this webinar, Dr. Erin Harberts, from Prof. Robert Ernst’s group at the University of Maryland, Baltimore will present her work on rationally designed toll like receptor 4 (TLR4) ligands for vaccine adjuvant discovery.

HLA-Profiled Cells & Tissues in Preclinical Research

In this webinar we will explain the basics of HLA genetics, give examples of applications for HLA genetic data, and explain how AllCells products can fulfill your research needs in the genomics era. Topics to be covered in the webinar: • An introduction to human HLA genetics and HLA typing • Why researchers use HLA-typed samples • Most common HLA types in populations • HLA-typed products at AllCells • Other immune response genetic profiles • Q&A

Primary Cells 101

Presented by AllCells® This webinar will introduce human and animal-derived primary cells and explain how they currently serve diverse biomedical clinical and research applications as biologically-relevant, species-specific, and simple to use biological systems. AllCells, your primary cells research partner, will be hosting this free webinar to help educate researchers. Join our Bioservices Scientists for this special engagement including a Q&A session immediately following the presentation. Topics to be covered in the webinar: Introduction: what are primary cells and how do they compare to cell lines Sources and types of primary cells Examples of primary cell applications How to work with primary cells Q&A

Maximize your cell culture Technical tips for primary cells and cell analyses

For over a decade, ScienCell Research Laboratories has helped researchers with their cell culture experiments by providing expert advice. Our webinar will discuss primary cell culture optimization techniques and why primary cells are necessary for validation of cell line studies and in vivo experiments. In addition, we will discuss how you can advance your primary cell research by using our gene expression profiling kits.

Optimizing qPCR sensitivity and specificity

qPCR can be a powerful tool for analyzing gene expression. When designed properly, however its strength relies on the quality of the primers used in it. Our scientists know this and follow stringent guidelines to design primers for our GeneQuery qPCR arrays. When researchers design their own primers without comprehensive knowledge of good primer design, qPCR results can be inadvertently influenced yielding unreliable and inconsistent data. Our webinar will cover guidelines used by our scientists in designing our quality GeneQuery qPCR primers arrays while highlighting our human endothelial cell biology array. An extended FAQ session for your questions and concerns will follow. Objectives: Delineate frequently overlooked primer optimization parameters during qPCR design. Demonstrate the benefits of properly optimized primers in qPCR analysis. Exemplify the ease and use of GeneQuery qPCR arrays in gene expression analysis.

StemBioSys : There is no place like home

The benefits of the unique CELLvo™ Matrix, the only natural cell culture substrate, will be discussed. The webinar describes the current challenges in removing cells from their native tissue and culturing them in a completely foreign environment such as tissue culture plastic. CELLvo™ Matrix addresses this by providing a “home” that mimics the native cell derived micro-environment that they came from. It promotes cell adhesion with better isolation and proliferation of primary cells and enhanced cellular responsiveness to experimental variables.

Flow Cytometry, Cell Culture

Using Flow Cytometry to Analyze Rare Hematopoietic Cell Subpopulations Implicated in Disease

Featuring our Bioservices team, this webinar on flow cytometry focuses on popular and high-demand flow cytometry assays utilized by the pharmaceutical and biotechnology sector in drug testing and disease modeling. Particular emphasis is on the hematopoietic system, its associated disease states, as well as immunophenotyping, cell functionality, drug efficacy, and rare-cell enrichment. Topics covered in the webinar: Review of Flow Cytometry Principles Immunophenotyping disease states and rare-cell subsets Cell Functionality: T-cell Polarization, B-cell Differentiation, and Neutrophil Migration Cell Enrichment: Nucleated Red Blood Cells, Rare T-cell subsets Q&A

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