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Cell Culture

Maximize your cell culture Technical tips for primary cells and cell analyses

For over a decade, ScienCell Research Laboratories has helped researchers with their cell culture experiments by providing expert advice. Our webinar will discuss primary cell culture optimization techniques and why primary cells are necessary for validation of cell line studies and in vivo experiments. In addition, we will discuss how you can advance your primary cell research by using our gene expression profiling kits.

Optimizing qPCR sensitivity and specificity

qPCR can be a powerful tool for analyzing gene expression. When designed properly, however its strength relies on the quality of the primers used in it. Our scientists know this and follow stringent guidelines to design primers for our GeneQuery qPCR arrays. When researchers design their own primers without comprehensive knowledge of good primer design, qPCR results can be inadvertently influenced yielding unreliable and inconsistent data. Our webinar will cover guidelines used by our scientists in designing our quality GeneQuery qPCR primers arrays while highlighting our human endothelial cell biology array. An extended FAQ session for your questions and concerns will follow. Objectives: Delineate frequently overlooked primer optimization parameters during qPCR design. Demonstrate the benefits of properly optimized primers in qPCR analysis. Exemplify the ease and use of GeneQuery qPCR arrays in gene expression analysis.

Making Cell Culture Relevant

Despite overwhelming evidence demonstrating the important role of the extracellular matrix in regulating cell behavior, traditional cell culture strategies do little to recreate a natural microenvironment. Presently, standard culture conditions inevitably lead to phenotypic drift, spontaneous differentiation, replicative senescence, and aberrant phenotypes that are unlike those found in the body. This may lead to experimental artifact in basic research or suboptimal clinical outcomes in translational research. To address this challenge, we focused our efforts on developing in vitro culture substrates that closely mimic natural microenvironments. Here we describe the development of scale-able, tissue-specific, cell-derived extracellular matrices for cell culture, and their applications in basic and translation research.

StemBioSys : There is no place like home

The benefits of the unique CELLvo Matrix, the only natural cell culture substrate, will be discussed. The webinar describes the current challenges in removing cells from their native tissue and culturing them in a completely foreign environment such as tissue culture plastic. CELLvo Matrix addresses this by providing a 'home' that mimics the native cell derived micro-environment that they came from. It promotes cell adhesion with better isolation and proliferation of primary cells and enhanced cellular responsiveness to experimental variables.

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