ProSci

APEX1 Antibody

Product Code:
 
PSI-13-943
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using APEX1 antibody (13-943).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human liver damage using APEX1 antibody (13-943) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using APEX1 antibody (13-943). Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of A549 cells using APEX1 antibody (13-943).
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Western blot analysis of extracts of various cell lines, using APEX1 antibody (13-943).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver damage using APEX1 antibody (13-943) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using APEX1 antibody (13-943). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cells using APEX1 antibody (13-943).

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PSI-13-943-50uL50uL£433.00
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Further Information

Additional Names:
APEX1, APE, APX, APE1, APEN, APEX, HAP1, REF1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1).
NCBI Gene ID #:
328
NCBI Official Name:
APEX nuclease (multifunctional DNA repair enzyme) 1
NCBI Official Symbol:
APEX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 39kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
P27695
User NOte:
Optimal dilutions for each application to be determined by the researcher.