ProSci

beta 2 Microglobulin Antibody, KO Validated

Product Code:
 
PSI-14-403
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using beta 2 Microglobulin antibody (14-403) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
2 / 4
Western blot analysis of extracts from normal (control) and beta 2 Microglobulin knockout (KO) 293T cells, using beta 2 Microglobulin antibody (14-403) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
3 / 4
Immunohistochemistry of paraffin-embedded rat brain using beta 2 Microglobulin antibody (14-403) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded human prostate using beta 2 Microglobulin antibody (14-403) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using beta 2 Microglobulin antibody (14-403) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Western blot analysis of extracts from normal (control) and beta 2 Microglobulin knockout (KO) 293T cells, using beta 2 Microglobulin antibody (14-403) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using beta 2 Microglobulin antibody (14-403) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using beta 2 Microglobulin antibody (14-403) at dilution of 1:100 (40x lens).

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PSI-14-403-50uL50uL£505.00
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Further Information

Additional Names:
B2M Antibody: Beta-2-microglobulin
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin (NP_004039.1).
NCBI Gene ID #:
567
NCBI Official Name:
beta-2-microglobulin
NCBI Official Symbol:
B2M
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 12kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
P61769
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.