ProSci

MKL1 Antibody

Product Code:
 
PSI-14-533
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using MKL1 antibody (14-533) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded rat brain using MKL1 antibody (14-533) (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using MKL1 antibody (14-533) (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using MKL1 antibody (14-533) (40x lens).
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Western blot analysis of extracts of various cell lines, using MKL1 antibody (14-533) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using MKL1 antibody (14-533) (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using MKL1 antibody (14-533) (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MKL1 antibody (14-533) (40x lens).

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PSI-14-533-50uL50uL£433.00
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Further Information

Additional Names:
MKL/myocardin-like protein 1, Megakaryoblastic leukemia 1 protein, Megakaryocytic acute leukemia protein, Myocardin-related transcription factor A, MRTF-A, MKL1, KIAA1438, MAL
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MKL1 (NP_065882.1).
NCBI Gene ID #:
57591
NCBI Official Name:
MKL/myocardin-like protein 1
NCBI Official Symbol:
MKL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 145kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Signal Transduction, Stem Cell, Transcription
Swissprot #:
Q969V6
User NOte:
Optimal dilutions for each application to be determined by the researcher.