ProSci

POLR1E Antibody

Product Code:
 
PSI-14-587
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using POLR1E antibody (14-587) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug POLR1E antibody (14-587). Western blot was performed from the immunoprecipitate using POLR1E antibody (14-587) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using POLR1E antibody (14-587) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug POLR1E antibody (14-587). Western blot was performed from the immunoprecipitate using POLR1E antibody (14-587) at a dilition of 1:1000.

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PSI-14-587-50uL50uL£433.00
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Further Information

Additional Names:
DNA-directed RNA polymerase I subunit RPA49, RNA polymerase I subunit A49, DNA-directed RNA polymerase I subunit E, RNA polymerase I-associated factor 1, RNA polymerase I-associated factor 53, POLR1E, PAF53, PRAF1
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human POLR1E (NP_071935.1).
NCBI Gene ID #:
64425
NCBI Official Name:
DNA-directed RNA polymerase I subunit RPA49
NCBI Official Symbol:
POLR1E
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 56kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9GZS1
User NOte:
Optimal dilutions for each application to be determined by the researcher.