ProSci

DDX58 Antibody, KO Validated

Product Code:
 
PSI-18-190
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts from normal (control) and DDX58 knockout (KO) A-549 cells, using DDX58 antibody (18-190) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1min.
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Western blot analysis of extracts from normal (control) and DDX58 knockout (KO) A-549 cells, using DDX58 antibody (18-190) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1min.

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PSI-18-190-50uL50uL£505.00
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Further Information

Additional Names:
RIG-1 Antibody: RIGI, RIG-I, RLR-1, Probable ATP-dependent RNA helicase DDX58, DEAD box protein 58
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:20 - 1:100

IP: 1:20 - 1:100
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 830 to the C-terminus of human DDX58 (NP_055129.2).
NCBI Gene ID #:
23586
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
NCBI Official Symbol:
DDX58
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 102kDa
Purification:
Affinity purification
Research Area:
Immunology
Swissprot #:
O95786
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.