ProSci

SNAIL Antibody

Product Code:
 
PSI-19-550
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using SNAIL antibody (19-550) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded human breast using SNAIL antibody (19-550) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human colon using SNAIL antibody (19-550) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of U2OS cells using SNAIL antibody (19-550) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using SNAIL antibody (19-550) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human breast using SNAIL antibody (19-550) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using SNAIL antibody (19-550) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SNAIL antibody (19-550) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-19-550-50uL50uL£433.00
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Further Information

Additional Names:
SNAI1, SNA, SNAH, SNAIL, SLUGH2, SNAIL1, dJ710H13.1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SNAIL (NP_005976.2).
NCBI Gene ID #:
6615
NCBI Official Name:
snail family zinc finger 1
NCBI Official Symbol:
Snai1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 29kDa
Purification:
Affinity purification
Research Area:
Cancer, Transcription
Swissprot #:
O95863
User NOte:
Optimal dilutions for each application to be determined by the researcher.