ProSci

GRIN1 Antibody

Product Code:
 
PSI-22-753
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using GRIN1 antibody (22-753).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunofluorescence analysis of rat brain using GRIN1 antibody (22-753) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of SKOV3 cells using GRIN1 antibody (22-753) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using GRIN1 antibody (22-753).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of rat brain using GRIN1 antibody (22-753) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of SKOV3 cells using GRIN1 antibody (22-753) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-22-753-50uL50uL£433.00
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Further Information

Additional Names:
GRIN1, glutamate receptor, ionotropic, N-methyl D-aspartate 1, NMDA1, NMDAR1, NR1, N-methyl-D-aspartate receptor channel, subunit zeta-1, NMDA receptor 1, glutamate [NMDA] receptor subunit zeta 1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 600-700 of human GRIN1 (NP_015566.1).
NCBI Gene ID #:
2902
NCBI Official Name:
glutamate receptor, ionotropic, N-methyl D-aspartate 1
NCBI Official Symbol:
GRIN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 105kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
Q05586
User NOte:
Optimal dilutions for each application to be determined by the researcher.