ProSci

ELF5 Antibody

Product Code:
 
PSI-22-761
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using ELF5 antibody (22-761) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of A549 cells using ELF5 antibody (22-761). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ELF5 antibody (22-761) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of A549 cells using ELF5 antibody (22-761). Blue: DAPI for nuclear staining.

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PSI-22-761-50uL50uL£433.00
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Further Information

Additional Names:
ELF5, E74-like factor 5 (ets domain transcription factor), ESE2, ESE-2, E74-like factor 5, epithelium-specific Ets transcription factor 2
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a member of an epithelium-specific subclass of the Ets transcritpion factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ELF5 (NP_001413.1).
NCBI Gene ID #:
2001
NCBI Official Name:
E74-like factor 5 (ets domain transcription factor)
NCBI Official Symbol:
ELF5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 31kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
Q9UKW6
User NOte:
Optimal dilutions for each application to be determined by the researcher.