ProSci

TNNC2 Antibody

Product Code:
 
PSI-23-146
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using TNNC2 antibody (23-146) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded human colon using TNNC2 antibody (23-146) at dilution of 1:200 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded mouse lung using TNNC2 antibody (23-146) at dilution of 1:200 (40x lens).
4 / 4
Immunofluorescence analysis of U2OS cells using TNNC2 antibody (23-146) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using TNNC2 antibody (23-146) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human colon using TNNC2 antibody (23-146) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using TNNC2 antibody (23-146) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using TNNC2 antibody (23-146) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-23-146-50uL50uL£433.00
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Further Information

Additional Names:
Troponin C, skeletal muscle, TNNC2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Troponin (Tn), a key protein complex in the regulation of striated muscle contraction, is composed of 3 subunits. The Tn-I subunit inhibits actomyosin ATPase, the Tn-T subunit binds tropomyosin and Tn-C, while the Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. The protein encoded by this gene is the Tn-C subunit.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human TNNC2 (NP_003270.1).
NCBI Gene ID #:
7125
NCBI Official Name:
Troponin C, skeletal muscle
NCBI Official Symbol:
TNNC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 18kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P02585
User NOte:
Optimal dilutions for each application to be determined by the researcher.