ProSci

GTF2I Antibody

Product Code:
 
PSI-23-264
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Immunofluorescence analysis of A431 cells using GTF2I antibody (23-264) at dilution of 1:100. Blue: DAPI for nuclear staining.
2 / 7
Immunohistochemistry of paraffin-embedded human esophagus using GTF2I antibody (23-264) (40x lens).
3 / 7
Immunohistochemistry of paraffin-embedded rat kidney using GTF2I antibody (23-264) (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using GTF2I antibody (23-264) (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using GTF2I antibody (23-264) (40x lens).
6 / 7
Western blot analysis of extracts of mouse brain, using GTF2I antibody (23-264) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
7 / 7
Western blot analysis of extracts of BT-474 cells, using GTF2I antibody (23-264) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of A431 cells using GTF2I antibody (23-264) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human esophagus using GTF2I antibody (23-264) (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using GTF2I antibody (23-264) (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using GTF2I antibody (23-264) (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using GTF2I antibody (23-264) (40x lens).
Western blot analysis of extracts of mouse brain, using GTF2I antibody (23-264) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of BT-474 cells, using GTF2I antibody (23-264) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

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CodeSizePrice
PSI-23-264-50uL50uL£433.00
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Further Information

Additional Names:
GTF2I, BAP-135, BAP135, BTKAP1, DIWS, IB291, SPIN, TFII-I, WBS, WBSCR6, GTFII-I
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human GTF2I
NCBI Gene ID #:
2969
NCBI Official Name:
general transcription factor IIi
NCBI Official Symbol:
GTF2I
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 132kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P78347
User NOte:
Optimal dilutions for each application to be determined by the researcher.