ProSci

ITPR1 Antibody

Product Code:
 
PSI-23-269
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using ITPR1 antibody (23-269) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of U-2 OS cells using ITPR1 antibody (23-269) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ITPR1 antibody (23-269) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U-2 OS cells using ITPR1 antibody (23-269) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-23-269-50uL50uL£433.00
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This product comes from: United States.
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Further Information

Additional Names:
ITPR1, InsP3R-I, IP3R1, InsP3R, InsP3R type I, INSP3R1, IP3R, Opisthotonus, Pcp1, IP3 receptor, IP3 receptor isoform 1, SCA15, Spinocerebellar ataxia 15, Spinocerebellar ataxia 16, I145TR, IP3R 1, SCA16, Type 1 InsP3 receptor
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Background:
This gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1850-1950 of human ITPR1 (XP_011531985.1).
NCBI Gene ID #:
3708
NCBI Official Name:
inositol 1,4,5-trisphosphate receptor, type 1
NCBI Official Symbol:
ITPR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 315kDa
Purification:
Affinity purification
Research Area:
Cancer, Immunology, Neuroscience, Signal Transduction
Swissprot #:
Q14643
User NOte:
Optimal dilutions for each application to be determined by the researcher.