ProSci

PSPH Antibody

Product Code:
 
PSI-23-282
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using PSPH antibody (23-282) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded human stomach using PSPH antibody (23-282) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded rat brain using PSPH antibody (23-282) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using PSPH antibody (23-282) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using PSPH antibody (23-282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PSPH antibody (23-282) at dilution of 1:100 (40x lens).

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PSI-23-282-50uL50uL£433.00
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Further Information

Additional Names:
PSPH, phosphoserine phosphatase, PSP, L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase, OTTHUMP00000025059, PSPase, PSPHD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human PSPH (NP_004568.2).
NCBI Gene ID #:
5723
NCBI Official Name:
phosphoserine phosphatase
NCBI Official Symbol:
PSPH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P78330
User NOte:
Optimal dilutions for each application to be determined by the researcher.