ProSci

MDC1 Antibody

Product Code:
 
PSI-23-531
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using MDC1 antibody (23-531) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate cancer using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U-2 OS cells using MDC1 antibody (23-531) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using MDC1 antibody (23-531) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using MDC1 antibody (23-531) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-23-531-50uL50uL£433.00
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Further Information

Additional Names:
IER3, NFBD1, KIAA0170, DKFZp781A0122
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1 (NP_055456.2).
NCBI Gene ID #:
9656
NCBI Official Name:
mediator of DNA-damage checkpoint 1
NCBI Official Symbol:
MDC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 270kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q14676
User NOte:
Optimal dilutions for each application to be determined by the researcher.