ProSci

GTF3C4 Antibody

Product Code:
 
PSI-23-830
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using GTF3C4 antibody (23-830) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunofluorescence analysis of U-2 OS cells using GTF3C4 Polyclonal Antibody (23-830) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using GTF3C4 antibody (23-830) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of U-2 OS cells using GTF3C4 Polyclonal Antibody (23-830) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-23-830-50uL50uL£433.00
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Further Information

Additional Names:
GTF3C4, general transcription factor IIIC, polypeptide 4 (90kD), OTTHUMP00000022434, TFiiiC2-90, TFIIICdelta, TFIIIC90, TFIII90, MGC138450, KAT12, FLJ21002, polypeptide 4, 90 kDa, GTF3C4, TFIIIC290, TFIIICDELTA
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 583-822 of human GTF3C4 (NP_036336.2).
NCBI Gene ID #:
9329
NCBI Official Name:
general transcription factor IIIC, polypeptide 4, 90kDa
NCBI Official Symbol:
GTF3C4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 95kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
Q9UKN8
User NOte:
Optimal dilutions for each application to be determined by the researcher.