ProSci

ABCB4 Antibody

Product Code:
 
PSI-23-933
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of mouse brain, using ABCB4 antibody (23-933) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Western blot analysis of extracts of mouse brain, using ABCB4 antibody (23-933) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

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PSI-23-933-50uL50uL£433.00
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This product comes from: United States.
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Further Information

Additional Names:
ABC21, GBD1, ICP3, MDR2, MDR2/3, MDR3, PFIC-3, PGY3, phosphatidylcholine translocator ABCB4, ATP-binding cassette sub-family B member 4, ATP-binding cassette, sub-family B (MDR/TAP), member 4, P-glycoprotein 3, P-glycoprotein-3/multiple drug resistance-3, multidrug resistance protein 3, multiple drug resistance 3
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human ABCB4 (NP_061337.1).
NCBI Gene ID #:
5244
NCBI Official Name:
ATP binding cassette subfamily B member 4
NCBI Official Symbol:
ABCB4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 144kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P21439
User NOte:
Optimal dilutions for each application to be determined by the researcher.