South Bay Bio

HA-Ubiquitin-vinyl methyl ester (human) (rec.) (HA)

Product Code:
 
SBB-PS0123
Product Group:
 
Recombinant Proteins
Supplier:
 
South Bay Bio
Regulatory Status:
 
RUO
Target Species:
 
Human
Shipping:
 
Dry Ice
Storage:
 
-80°C
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SBB-PS0123-C05050 ug£354.00
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Further Information

Alternate Names/Synonyms:
HA-Ub-VME; HA-Ubiquitin-VME; HA-Ubiquitin-Vinyl methyl ester
Concentration:
Lot dependent.
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 50mM MES pH 6.0, 100mM sodium chloride.
Handling Advice:
Aliquot to avoid freeze/thaw cycles.
Long Description:
Protein. Human ubiquitin (aa1-76) (Accession Nr. P0CG47) with a C-terminal vinyl methyl ester group and a N-terminal HA-Tag. Source: E. coli. Liquid. In 50mM MES pH 6.0, 100mM sodium chloride. Purity: >97% (LCMS). Ubiquitin is a small (8.5kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation. Ubiquitination can affect proteins in many ways: it can signal for their degradation via the proteasome, alter their cellular location, affect their activity and promote or prevent protein interactions. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various linkage specificities. This product consists of a full-length human, mature ubiquitin polypeptide (amino acids 1-76), expressed in E.coli with a C-terminal warhead (Vinyl Methyl Ester) and an N-terminal HA-Tag. HA-Ubiquitin vinyl methyl ester is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl methyl ester group. It targets four of the five major DUB families: UCH (Ubiquitin C-terminal hydrolases), USP (Ubiquitin specific proteases), OTU (Ovarian tumor proteases) and MJD (Machado-Josephin domain proteases) while JAMM metalloproteases are not inhibited. HA-Ubiquitin vinyl methyl ester can be used for activity profiling experiments and using the HA-Tag conveniently for Immunoprecipitations in search for new or novel enzymes reacting with the c-terminal warhead.
Molecular Weight:
~10kDa
NCBI, Uniprot Number:
P0CG47
Package Type:
Plastic Vial
Product Description:
Ubiquitin is a small (8.5kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation. Ubiquitination can affect proteins in many ways: it can signal for their degradation via the proteasome, alter their cellular location, affect their activity and promote or prevent protein interactions. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various linkage specificities. This product consists of a full-length human, mature ubiquitin polypeptide (amino acids 1-76), expressed in E.coli with a C-terminal warhead (Vinyl Methyl Ester) and an N-terminal HA-Tag. HA-Ubiquitin vinyl methyl ester is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl methyl ester group. It targets four of the five major DUB families: UCH (Ubiquitin C-terminal hydrolases), USP (Ubiquitin specific proteases), OTU (Ovarian tumor proteases) and MJD (Machado-Josephin domain proteases) while JAMM metalloproteases are not inhibited. HA-Ubiquitin vinyl methyl ester can be used for activity profiling experiments and using the HA-Tag conveniently for Immunoprecipitations in search for new or novel enzymes reacting with the c-terminal warhead.
Purity:
>97% (LCMS)
Sequence:
Human ubiquitin (aa1-76) (Accession Nr. P0CG47 http://www.ncbi.nlm.nih.gov/protein/P0CG47 ) with a C-terminal vinyl methyl ester group and a N-terminal HA-Tag.
Source / Host:
E. coli
TAGs:
HA
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -80°C.