ProSci

SARS-CoV-2 Spike P26S Antibody (Gamma Variant)

Product Code:
 
PSI-9573
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
blue ice or RT
Storage:
 
SARS-CoV-2 Spike P26S antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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<strong>Figure 1 SARS-Cov-2 Spike P26S ( Gamma Variant ) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in an ELISA </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, 9573, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.
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<strong>Figure 2 ELISA Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT and Gamma variant (P.1), 1 μg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, 9573, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. <strong>SARS-CoV-2 Spike P26S (Gamma Variant) antibody (9573) specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (10-300). </strong>
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<strong>Figure 3 WB Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein </strong><br> Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Gamma variant (P.1). Detection Antibodies: Spike P26S (Gamma Variant) antibody, 9573, 1 μg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. <strong>SARS-CoV-2 Spike P26S (Gamma Variant) antibody (9573) specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (10-300). </strong>
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<strong>Figure 1 SARS-Cov-2 Spike P26S ( Gamma Variant ) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in an ELISA </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, 9573, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.
<strong>Figure 2 ELISA Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT and Gamma variant (P.1), 1 μg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, 9573, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. <strong>SARS-CoV-2 Spike P26S (Gamma Variant) antibody (9573) specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (10-300). </strong>
<strong>Figure 3 WB Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein </strong><br> Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Gamma variant (P.1). Detection Antibodies: Spike P26S (Gamma Variant) antibody, 9573, 1 μg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. <strong>SARS-CoV-2 Spike P26S (Gamma Variant) antibody (9573) specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (10-300). </strong>

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PSI-9573-0.02mg0.02mg£150.00
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PSI-9573-0.1mg0.1mg£449.00
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Further Information

Additional Names:
SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Application Note:
WB: 1 μg/mL.

Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects SARS-CoV-2 Gamma Variant (P.1) Spike S1 protein, but not SARS-CoV-2 WT and other variant Spike S1 protein by ELISA. All other applications and species not yet tested.
Background:
In January of 2021 a new lineage of SARS-CoV-2, known as P.1 and named as Gamma variant, was discovered in Japan and later spread in Brazil. It is considered as VOC (variant of concern). This variant carries 10 mutations in spike protein, including N501Y, E484K and K417T in RBD, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Gamma variant (P.1 lineage) was observed globally, which is 3.5 times more contagious as the original one. The Gamma variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent due to the immune escape E484K mutation.
Background References:
  • Voloch et al. Journal of Virology 2021, 95 (10): e00119-21.
  • Wang et al. Cell Host & Microbe 2021, 29(5): 747-751.
  • Wang et al. Nature 592, 616-622.
Buffer:
SARS-CoV-2 Spike P26S antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (36.4%)
Immunogen:
Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody (9573) was raised against a peptide corresponding to 11 amino acids near the amino terminus of SARS-CoV-2 Gamma variant (P.1) Spike protein.

The immunogen is located within the first 50 amino acids of SARS-CoV-2 Gamma variant (P.1) Spike protein.
NCBI Gene ID #:
43740568
NCBI Official Name:
surface glycoprotein
NCBI Official Symbol:
S
NCBI Organism:
SARS-CoV-2
Physical State:
Liquid
Protein Accession #:
QHD43416
Protein GI Number:
1791269090
Purification:
SARS-CoV-2 Spike P26S (Gamma Variant) antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
SPECIFICITY:
It can only detect SARS-CoV-2 Gamma Variant (P.1) Spike S1 protein and does not cross-react with the spike protein of other variants.
Swissprot #:
P0DTC2
User NOte:
Optimal dilutions for each application to be determined by the researcher.