NSJ Bioreagents

b-Actin Antibody

Product Code:
 
NSJ-F40216
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, k
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
137CT26.1.1
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the b-Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot analysis of b-Actin antibody in 1) K562, 2) HL-60, 3) HeLa cell line, and mouse tissues 4) spleen, 5) liver, 6) mouse NIH3T3 cell lysate, 7) mouse cerebellum and 8) mouse brain tissue lysate
2 / 3
Immunohistochemical analysis of paraffin-embedded human spleen using b-Actin antibody at 1:25 dilution.
3 / 3
Confocal immunofluorescent analysis of b-Actin antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).
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Western blot analysis of b-Actin antibody in 1) K562, 2) HL-60, 3) HeLa cell line, and mouse tissues 4) spleen, 5) liver, 6) mouse NIH3T3 cell lysate, 7) mouse cerebellum and 8) mouse brain tissue lysate
Immunohistochemical analysis of paraffin-embedded human spleen using b-Actin antibody at 1:25 dilution.
Confocal immunofluorescent analysis of b-Actin antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).

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CodeSizePrice
NSJ-F40216-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40216-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Western blot: 1:1000
Application Note:
Titration of the b-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus. It is classically referred to as being a 'nonmuscle' actin, however expression is ubiquitous, strongly in lymph tissue and weakly in heart and muscle.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This b-Actin antibody was produced from a mouse immunized with ACTB recombinant protein.
Limitation:
This b-Actin antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat, Bovine, Pig, Primate, Chicken, Xenopus
Uniprot #:
P60709