NSJ Bioreagents

CHRM2 Antibody

Product Code:
 
NSJ-F54335
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
1424CT461.78.60
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the CHRM2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC testing of FFPE human brain tissue with CHRM2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human heart tissue with CHRM2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Immunofluorescent staining of fixed and permeabilized human SH-SY5Y cells with CHRM2 antibody (green) and DAPI nuclear stain (blue).
4 / 5
Western blot testing of 1) human SH-SY5Y, 2) human brain and 3) mouse brain lysate with CHRM2 antibody. Predicted molecular weight ~52 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with CHRM2 antibody; Blue=isotype control, Green= CHRM2 antibody.
❮❯

IHC testing of FFPE human brain tissue with CHRM2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human heart tissue with CHRM2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human SH-SY5Y cells with CHRM2 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of 1) human SH-SY5Y, 2) human brain and 3) mouse brain lysate with CHRM2 antibody. Predicted molecular weight ~52 kDa.
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with CHRM2 antibody; Blue=isotype control, Green= CHRM2 antibody.

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CodeSizePrice
NSJ-F54335-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54335-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the CHRM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A recombinant human protein was used as the immunogen for the CHRM2 antibody.
Limitation:
This CHRM2 antibody is available for research use only.
Purity:
Protein G affinity
Species Reactivity :
Human, Mouse
Uniprot #:
P08172