NSJ Bioreagents

ALDH6A1 Antibody

Product Code:
 
NSJ-F40222
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, k
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
147CT8.3.4
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the ALDH6A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Fluorescent image of MCF-7 cells stained with ALDH6A1 antibody; Ab was diluted at 1:25 dilution. An Alexa Fluor 488 secondary Ab (green); DAPI counterstain (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
2 / 4
Immunohistochemical analysis of paraffin-embedded human colon section using ALDH6A1 antibody at 1:25 dilution.
3 / 4
Western blot analysis of lysate from MCF-7, T47D cell line (left to right) using ALDH6A1 antibody at 1:1000 for each lane.
4 / 4
Immunohistochemical analysis of paraffin-embedded human liver section using ALDH6A1 antibody at 1:25 dilution.
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Fluorescent image of MCF-7 cells stained with ALDH6A1 antibody; Ab was diluted at 1:25 dilution. An Alexa Fluor 488 secondary Ab (green); DAPI counterstain (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded human colon section using ALDH6A1 antibody at 1:25 dilution.
Western blot analysis of lysate from MCF-7, T47D cell line (left to right) using ALDH6A1 antibody at 1:1000 for each lane.
Immunohistochemical analysis of paraffin-embedded human liver section using ALDH6A1 antibody at 1:25 dilution.

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CodeSizePrice
NSJ-F40222-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40222-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunofluorescence: 1:25,IHC (Paraffin): 1:25,Western blot: 1:1000
Application Note:
Titration of the ALDH6A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This protein belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. The product of this gene, a mitochondrial methylmalonate semialdehyde dehydrogenase, catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This ALDH6A1 antibody was produced from a mouse immunized with ALDH6A1 recombinant protein.
Limitation:
This ALDH6A1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q02252