NSJ Bioreagents

SOX2 Antibody

Product Code:
 
NSJ-F40361
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
57CT23.3.4
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the SOX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 6
Western blot analysis of lysate from NCCIT cell line using SOX2 antibody at 1:1000.
2 / 6
Western blot analysis of SOX2 recombinant protein using SOX2 antibody at 1:4000.
3 / 6
Fluorescent image of A549 cell stained with SOX2 antibody at 1:25. Immunoreactivity is localized to the nucleus.
4 / 6
Western blot analysis of SOX2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (2)
5 / 6
IHC analysis of FFPE human lung carcinoma tissue stained with SOX2 antibody
6 / 6
Flow cytometric analysis of NCI-H460 cells using SOX2 antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.
❮❯

Western blot analysis of lysate from NCCIT cell line using SOX2 antibody at 1:1000.
Western blot analysis of SOX2 recombinant protein using SOX2 antibody at 1:4000.
Fluorescent image of A549 cell stained with SOX2 antibody at 1:25. Immunoreactivity is localized to the nucleus.
Western blot analysis of SOX2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (2)
IHC analysis of FFPE human lung carcinoma tissue stained with SOX2 antibody
Flow cytometric analysis of NCI-H460 cells using SOX2 antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-F40361-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40361-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Immunofluorescence: 1:10-1:50,Western blot: 1:200-1:2000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the SOX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
recombinant protein was used to produce this monoclonal SOX2 antibody.
Limitation:
This SOX2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P48431