BRCA1 Antibody (Breast Marker)

Immunohistochemistry (FFPE) : 2-5ug/ml for 60 min at RT

Optimal dilution of the BRCA1 antibody should be determined by the researcher.

BRCA1 (Breast cancer type 1 susceptibility protein) is a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Amino acids 445-620 were used as the immunogen for the BRCA1 antibody.

This BRCA1 antibody is available for research use only.

Nuclear, cytoplasmic

Protein G affinity chromatography

Human

P38398