NSJ Bioreagents

EpCAM Antibody

Product Code:
 
NSJ-V8217
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
EGP40/2571R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
Flow cytometry testing of human MCF7 cells with recombinant EpCAM antibody (clone EGP40/2571R); Red=isotype control, Blue= recombinant EpCAM antibody.
2 / 4
IHC staining of FFPE human prostate carcinoma with recombinant EpCAM antibody (clone EGP40/2571R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE human colon carcinoma with recombinant EpCAM antibody (clone EGP40/2571R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
4 / 4
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/2571R) as confirmation of integrity and purity.
❮❯

Flow cytometry testing of human MCF7 cells with recombinant EpCAM antibody (clone EGP40/2571R); Red=isotype control, Blue= recombinant EpCAM antibody.
IHC staining of FFPE human prostate carcinoma with recombinant EpCAM antibody (clone EGP40/2571R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with recombinant EpCAM antibody (clone EGP40/2571R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/2571R) as confirmation of integrity and purity.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V8217-20UG20 ug£264.00
Quantity:
NSJ-V8217-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells in 0.1ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human partial protein (amino acids 100-224) was used as the immunogen for the EpCAM antibody. The epitope of this mAb has been mapped to between amino acids 202-212.
Limitation:
This recombinant EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P16422