NSJ Bioreagents

GFAP Antibody / Glial Fibrillary Acidic Protein

Product Code:
 
NSJ-V3705
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
GFA12-4R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant GFAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of FFPE human cerebellum tissue with recombinant GFAP antibody (clone GFA12-4R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human cerebellum tissue with recombinant GFAP antibody (clone GFA12-4R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

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CodeSizePrice
NSJ-V3705-20UG20 ug£264.00
Quantity:
NSJ-V3705-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant GFAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Glial fibrillary acidic protein is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [RefSeq]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Pig spinal cord GFAP was used as the immunogen for this recombinant GFAP antibody.
Limitation:
This recombinant GFAP antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P14136