NSJ Bioreagents

Insulin Receptor alpha Antibody

Product Code:
 
NSJ-V3586
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
INSR/2277R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant Insulin Receptor alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC testing of FFPE human placental tissue with recombinant Insulin Receptor alpha antibody (clone INSR/2277R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.~
2 / 2
SDS-PAG

IHC testing of FFPE human placental tissue with recombinant Insulin Receptor alpha antibody (clone INSR/2277R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.~
SDS-PAG

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CodeSizePrice
NSJ-V3586-20UG20 ug£264.00
Quantity:
NSJ-V3586-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant Insulin Receptor alpha antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
The insulin receptor (INSR) is a heterodimeric protein complex that has an intracellular beta subunit and an extracellular a subunit, which is disulfide-linked to a transmembrane segment. The insulin ligand binds to the INSR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to INSR induces phosphorylation of intra-cellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin. Type 1 diabetes is an autoimmune condition of the endocrine pancreas that results in destruction of insulin secreting cells and a progressive loss in insulin-sensitive glucose uptake by cells.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A recombinant partial protein from the extracellular region of the protein was used as the immunogen for the recombinant Insulin Receptor alpha antibody.
Limitation:
This recombinant Insulin Receptor alpha antibody is available for research use only.
Localization:
Cytoplasm, cell membrane
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P06213