NSJ Bioreagents

p53 Antibody / TP53 (C-Terminal Region)

Product Code:
 
NSJ-V3510
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PAb 122
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Store the p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 1
Protein array validation of the p53 antibody: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using p53 antibody (clone PAb 122).<P><P>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-Ig

Protein array validation of the p53 antibody: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using p53 antibody (clone PAb 122).<P><P>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-Ig

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V3510-20UG20 ug£264.00
Quantity:
NSJ-V3510-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
ELISA (order BSA/sodium azide-free format for coating)
Application Note:
Variations in protocols, secondaries and substrates may require the p53 antibody to be titered up or down for optimal performance.
Description:
This antibody is specific to a conserved determinant of the p53 protein. PAb 122 binds to the C-terminus (aa 370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb 122 blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). The protein in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Its the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
7157
Immunogen:
SV40-transformed mouse B4 cells were used as the immunogen for this antibody. Its epitope maps near the C-terminal end (aa 370-378) of p53.
Limitation:
This p53 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse, Rat