NSJ Bioreagents

H4K20me3 Antibody / Trimethyl Histone H4 Lysine 20

Product Code:
 
NSJ-R20238
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM208
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Store the recombinant H4K20me3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Western blot test of (1) acid extracts of HeLa cells and (2) recombinant Histone H4, using recombinant H4K20me3 antibody at 1 ug/ml, showed a band of Histone H4 trimethylated at Lysine 20 in HeLa cells.~
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This recombinant H4K20me3 antib
❮❯

Western blot test of (1) acid extracts of HeLa cells and (2) recombinant Histone H4, using recombinant H4K20me3 antibody at 1 ug/ml, showed a band of Histone H4 trimethylated at Lysine 20 in HeLa cells.~
This recombinant H4K20me3 antib

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CodeSizePrice
NSJ-R20238-25UG25ug£321.00
Quantity:
NSJ-R20238-100UG100ug£581.00
Quantity:
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Further Information

Application Details :
Western blot: 1-2ug/ml,ELISA: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H4K20me3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant H4K20me3 antibody reacts to Histone H4 trimethylated at Lysine 20 (K20me3). It may slightly cross react to dimethylated Lysine 20 (K20me2) at a higher concentration. No cross reactivity with non-modified or monomethylated Lysine 20 (K20me1), or other methylations in histone H4.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A trimethyl-peptide corresponding to Trimethyl-Histone H4 (Lys20) was used as the immunogen for this recombinant H4K20me3 antibody.
Limitation:
This recombinant H4K20me3 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P62805