NSJ Bioreagents

H2BK20ac Antibody / Acetyl Histone H2B Lysine 20

Product Code:
 
NSJ-R20241
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM235
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
 
Store the recombinant H2BK20ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
1 / 3
Western blot test of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using recombinant H2BK20ac antibody at 0.5 ug/ml.
2 / 3
This recombinant H2BK20ac antibody specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in Histone H2B.
3 / 3
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H2BK20ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
❮❯

Western blot test of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using recombinant H2BK20ac antibody at 0.5 ug/ml.
This recombinant H2BK20ac antibody specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in Histone H2B.
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H2BK20ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

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CodeSizePrice
NSJ-R20241-25UG25ug£321.00
Quantity:
NSJ-R20241-100UG100ug£581.00
Quantity:
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Further Information

Application Details :
Western blot: 0.5-2ug/ml,ELISA: 0.2-1ug/ml,Immunocytochemistry: 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H2BK20ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant H2BK20ac antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20) was used as the immunogen for this recombinant H2BK20ac antibody.
Limitation:
This recombinant H2BK20ac antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
Human
Uniprot #:
P33778