NSJ Bioreagents

NGF Receptor Antibody / p75NTR / CD271

Product Code:
 
NSJ-V7975
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rNGFR/1965
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant NGF Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
Western blot testing of human U-87 cell lysate with recombinant NGF Receptor antibody (clone rNGFR/1965). Predicted molecular weight: 45-75 kDa depending on glycosylation level.
2 / 4
IHC staining of FFPE human melanoma with recombinant NGF Receptor antibody (clone rNGFR/1965). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE human melanoma with recombinant NGF Receptor antibody (clone rNGFR/1965). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
SDS-PAGE analysis of purified, BSA-free recombinant NGF Receptor antibody (clone rNGFR/1965) as confirmation of integrity and purity.
❮❯

Western blot testing of human U-87 cell lysate with recombinant NGF Receptor antibody (clone rNGFR/1965). Predicted molecular weight: 45-75 kDa depending on glycosylation level.
IHC staining of FFPE human melanoma with recombinant NGF Receptor antibody (clone rNGFR/1965). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human melanoma with recombinant NGF Receptor antibody (clone rNGFR/1965). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant NGF Receptor antibody (clone rNGFR/1965) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V7975-20UG20 ug£264.00
Quantity:
NSJ-V7975-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml (not suitable for frozen sample testing),Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human partial protein (amino acids 281-421) was used as the immunogen for this recombinant NGF Receptor antibody.
Limitation:
This recombinant NGF Receptor antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P08138