NSJ Bioreagents

Villin Antibody

Product Code:
 
NSJ-V3566
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rVIL1/1325
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant Villin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of FFPE human small intestine carcinoma with recombinant Villin antibody (clone rVIL1/1325). Required HIER: boil tissue sections in pH9 EDTA buffer, for 10-20 min followed by cooling at RT for 20 min.~
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IHC testing of FFPE hum
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IHC testing of FFPE human small intestine carcinoma with recombinant Villin antibody (clone rVIL1/1325). Required HIER: boil tissue sections in pH9 EDTA buffer, for 10-20 min followed by cooling at RT for 20 min.~
IHC testing of FFPE hum

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CodeSizePrice
NSJ-V3566-20UG20 ug£264.00
Quantity:
NSJ-V3566-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Villin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Amino acids 179-311 from the human protein were used as the immunogen for this recombinant Villin antibody.
Limitation:
This recombinant Villin antibody is available for research use only.
Localization:
Cytoplasmic and cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P09327