NSJ Bioreagents

Phospho c-Myc Antibody (pT58)

Product Code:
 
NSJ-F48482
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot the phospho c-Myc antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Western blot analysis of phospho c-Myc antibody and human TPA activated HeLa cells/lysate (0: without TPA; 1: 60ug/ml TPA-15min; 2: 60ug/ml-30min; 3: 60ug/ml-45min; 4: 125ug/ml-15min; 5: 125ug/ml-30min; 6: 125ug/ml-45min; 7: 250ug/ml-15min; 8: 250ug/ml-30min; 9: 250ug/ml-45min)~
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Western blot analysis of phospho c-Myc antibody and human TPA activated HeLa cells/lysate (0: without TPA; 1: 60ug/ml TPA-15min; 2: 60ug/ml-30min; 3: 60ug/ml-45min; 4: 125ug/ml-15min; 5: 125ug/ml-30min; 6: 125ug/ml-45min; 7: 250ug/ml-15min; 8: 250ug/ml-30min; 9: 250ug/ml-45min)~

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CodeSizePrice
NSJ-F48482-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48482-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Dot blot: 1:500,Western blot: 1:1000
Application Note:
Titration of the phospho c-Myc antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
c-Myc participates in the regulation of gene transcription. It binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. This protein appears to activate the transcription of growth-related genes. Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho c-Myc antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT58 of human MYC.
Limitation:
This phospho c-Myc antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Bovine, Chicken, Mouse, Pig, Rat, Xenopus, Zebrafish
Uniprot #:
P01106