NSJ Bioreagents

Phospho-Histone H3 Antibody (pS10)

Product Code:
 
NSJ-F48401
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the phospho-Histone H3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 9
Western blot analysis of lysate from HeLa cell line, untreated or treated with Calyculin A (100ng/ml), using phospho-Histone H3 antibody
2 / 9
Western blot analysis of lysate from HeLa cell line, untreated or treated with FBS + Calyculin A (100ng/ml), using phospho-Histone H3 antibody
3 / 9
Western blot analysis of lysate from HeLa cell line using phospho-Histone H3 antibody at 1:1000.
4 / 9
Western blot analysis of extracts from HeLa cells, untreated or treated with FBS + Calyculin A (100nM), using phospho-Histone H3 antibody (left) or nonphos Ab (right).
5 / 9
Western blot analysis of extracts from HeLa cells, untreated or treated with FBS and Calyculin A (100nM), using phospho-Histone H3 antibody (left) or nonphos Ab (right).
6 / 9
Dot blot analysis of phospho-Histone H3 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
7 / 9
IHC staining of FFPE human tonsil with phospho-Histone H3 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
8 / 9
IHC staining of FFPE human breast cancer with phospho-Histone H3 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
9 / 9
Immunofluorescent staining of PFA fixed and permeabilized human HeLa cells with phospho-Histone H3 antibody (green) and Phalloidin (red).
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Western blot analysis of lysate from HeLa cell line, untreated or treated with Calyculin A (100ng/ml), using phospho-Histone H3 antibody
Western blot analysis of lysate from HeLa cell line, untreated or treated with FBS + Calyculin A (100ng/ml), using phospho-Histone H3 antibody
Western blot analysis of lysate from HeLa cell line using phospho-Histone H3 antibody at 1:1000.
Western blot analysis of extracts from HeLa cells, untreated or treated with FBS + Calyculin A (100nM), using phospho-Histone H3 antibody (left) or nonphos Ab (right).
Western blot analysis of extracts from HeLa cells, untreated or treated with FBS and Calyculin A (100nM), using phospho-Histone H3 antibody (left) or nonphos Ab (right).
Dot blot analysis of phospho-Histone H3 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
IHC staining of FFPE human tonsil with phospho-Histone H3 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with phospho-Histone H3 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of PFA fixed and permeabilized human HeLa cells with phospho-Histone H3 antibody (green) and Phalloidin (red).

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CodeSizePrice
NSJ-F48401-0.05ML0.05 ml£260.00
Quantity:
NSJ-F48401-0.2ML0.2 ml£502.00
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Further Information

Application Details :
Western blot: 1:500-4000,Immunohistochemistry (FFPE): 1:-50-1:100,Immunofluorescence: 1:25,Dot blot: 1:500
Application Note:
Titration of the phospho-Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-Histone H3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS10 of human HIST1H3A.
Limitation:
This phospho-Histone H3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P68431