NSJ Bioreagents

FGR Antibody / c-FGR proto oncogene

Product Code:
 
NSJ-F54446
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the FGR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human Raji cell lysate with FGR antibody. Predicted molecular weight: ~59 kDa, routinely observed at 55~59 kDa.
2 / 4
Western blot testing of (left) non-transfected and (right) transfected 293 cell lysate with FGR antibody.
3 / 4
Immunofluorescent staining of human MDA-MB-231 cells with FGR antibody (green) and DAPI nuclear stain (blue).
4 / 4
IHC testing of FFPE human breast cancer tissue with FGR antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Western blot testing of human Raji cell lysate with FGR antibody. Predicted molecular weight: ~59 kDa, routinely observed at 55~59 kDa.
Western blot testing of (left) non-transfected and (right) transfected 293 cell lysate with FGR antibody.
Immunofluorescent staining of human MDA-MB-231 cells with FGR antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human breast cancer tissue with FGR antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54446-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54446-0.4ML0.4 ml£502.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Immunofluorescence: 1:25,Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the FGR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
FGR is a member of the Src family of protein tyrosine kinases (PTKs). This protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. It localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this protein.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 3-33 from the human protein was used as the immunogen for the FGR antibody.
Limitation:
This FGR antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
SAS precipitation
Species Reactivity :
Human
Uniprot #:
P09769