NSJ Bioreagents

HSP60 Antibody

Product Code:
 
NSJ-F54512
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the 60 kDa Heat Shock Protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human 1) Jurkat, 2) HeLa, 3) HepG2, 4) MCF7, 5) mouse NIH 3T3 and 6) rat PC-12 cell lysate with 60 kDa Heat Shock Protein antibody. Predicted molecular weight ~60 kDa.
2 / 4
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with 60 kDa Heat Shock Protein antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
3 / 4
IHC testing of FFPE human lung adenocarcinoma tissue with 60 kDa Heat Shock Protein antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of fixed and permeabilized human HeLa cells with 60 kDa Heat Shock Protein antibody; Blue=isotype control, Green= 60 kDa Heat Shock Protein antibody.
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Western blot testing of human 1) Jurkat, 2) HeLa, 3) HepG2, 4) MCF7, 5) mouse NIH 3T3 and 6) rat PC-12 cell lysate with 60 kDa Heat Shock Protein antibody. Predicted molecular weight ~60 kDa.
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with 60 kDa Heat Shock Protein antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human lung adenocarcinoma tissue with 60 kDa Heat Shock Protein antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human HeLa cells with 60 kDa Heat Shock Protein antibody; Blue=isotype control, Green= 60 kDa Heat Shock Protein antibody.

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CodeSizePrice
NSJ-F54512-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54512-0.2ML0.2 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the 60 kDa Heat Shock Protein antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the 60 kDa Heat Shock Protein antibody.
Limitation:
This 60 kDa Heat Shock Protein antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P10809