NSJ Bioreagents

ERAB Antibody / HSD17B10

Product Code:
 
NSJ-F54513
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the HSD17B10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human 1) HeLa, 2) Jurkat, 3) K562, 4) HepG2, 5) SH-SY5Y, 6) rat brain and 7) mouse brain lysate with HSD17B10 antibody. Predicted molecular weight ~27 kDa.
2 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with HSD17B10 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
3 / 4
IHC testing of FFPE human thyroid carcinoma tissue with HSD17B10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of fixed and permeabilized human HeLa cells with HSD17B10 antibody; Blue=isotype control, Green= HSD17B10 antibody.
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Western blot testing of human 1) HeLa, 2) Jurkat, 3) K562, 4) HepG2, 5) SH-SY5Y, 6) rat brain and 7) mouse brain lysate with HSD17B10 antibody. Predicted molecular weight ~27 kDa.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with HSD17B10 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human thyroid carcinoma tissue with HSD17B10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human HeLa cells with HSD17B10 antibody; Blue=isotype control, Green= HSD17B10 antibody.

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CodeSizePrice
NSJ-F54513-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54513-0.2ML0.2 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the HSD17B10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. Catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. Catalyzes the third step in the beta-oxidation of fatty acids. Carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids. Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the HSD17B10 antibody.
Limitation:
This HSD17B10 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q99714