NSJ Bioreagents

KRT12 Antibody / Cytokeratin 12

Product Code:
 
NSJ-F54280
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Cytokeratin 12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC testing of FFPE human colon carcinoma tissue with Cytokeratin 12 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
Western blot testing of human Y79 cell lysate with Cytokeratin 12 antibody. Predicted molecular weight ~54 kDa.
3 / 5
Western blot testing of mouse eyeball lysate with Cytokeratin 12 antibody. Predicted molecular weight ~54 kDa.
4 / 5
Western blot testing of rat eyeball lysate with Cytokeratin 12 antibody. Predicted molecular weight ~54 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human K562 cells with Cytokeratin 12 antibody; Blue=isotype control, Green= Cytokeratin 12 antibody.
❮❯

IHC testing of FFPE human colon carcinoma tissue with Cytokeratin 12 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human Y79 cell lysate with Cytokeratin 12 antibody. Predicted molecular weight ~54 kDa.
Western blot testing of mouse eyeball lysate with Cytokeratin 12 antibody. Predicted molecular weight ~54 kDa.
Western blot testing of rat eyeball lysate with Cytokeratin 12 antibody. Predicted molecular weight ~54 kDa.
Flow cytometry testing of fixed and permeabilized human K562 cells with Cytokeratin 12 antibody; Blue=isotype control, Green= Cytokeratin 12 antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-F54280-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54280-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Cytokeratin 12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 442-471 from the human protein were used as the immunogen for the Cytokeratin 12 antibody.
Limitation:
This Cytokeratin 12 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q99456