ELK Biotechnology

Human HVD3(25-Hydroxyvitamin D3) ELISA Kit

Product Code:
 
ELK8625
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

No additional charges, what you see is what you pay! *

CodeSizePrice
ELK8625-48T48T£261.00
Quantity:
ELK8625-96T96T£335.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Videos
  • Further Information
  • Show All

Video

Further Information

Alternative Names:
25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
3.13-200 ng/mL
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
0.91 ng/mL
Standard:
200 ng/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HVD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.