ELK Biotechnology

Human sCD14(soluble cluster of differentiation 14) ELISA Kit

Product Code:
 
ELK8435
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK8435-48T48T£298.00
Quantity:
ELK8435-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
CD14 Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD antigen CD14; soluble cluster of differentiation 14
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.32-20 ng/mL
Sample Type:
serum, plasma, cell lysates and other biological fluids
Sensitivity:
0.229 ng/mL
Standard:
20 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sCD14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sCD14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sCD14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human sCD14 in the samples is then determined by comparing the OD of the samples to the standard curve.