ELK Biotechnology

Human cGMP(Cyclic Guanosine Monophosphate) ELISA Kit

Product Code:
 
ELK8617
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK8617-48T48T£261.00
Quantity:
ELK8617-96T96T£335.00
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This product comes from: China.
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Further Information

Alternative Names:
c-GMP; 3',5'-Cyclic GMP; Cyclic 3',5'-GMP; Guanosine 3',5'-Cyclic Phosphate
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
156.25-10000 pg/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
45.2 pg/mL
Standard:
10000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human cGMP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human cGMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human cGMP in the samples is then determined by comparing the OD of the samples to the standard curve.