ELK Biotechnology

Human C5b(Complement Fragment 5b) ELISA Kit

Product Code:
 
ELK8530
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK8530-48T48T£298.00
Quantity:
ELK8530-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
Complement Fragment 5b
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.79-50 ng/mL
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
0.459 ng/mL
Standard:
50 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human C5b in the samples is then determined by comparing the OD of the samples to the standard curve.